tailieunhanh - Báo cáo y học: " Hepatitis C virus infection in Brazilian long-distance truck drivers"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus infection in Brazilian long-distance truck drivers | Freitas et al. Virology Journal 2010 7 205 http content 7 1 205 VIROLOGY JOURNAL SHORT REPORT Open Access Hepatitis C virus infection in Brazilian long-distance truck drivers 2 2 2 1 3 Nara R Freitas Sheila A Teles Marcos A Matos Carmen LR Lopes Nádia RS Reis Márcia P Espírito-Santo Elisabeth Lampe3 Regina MB Martins1 Abstract Hepatitis C virus HCV infection is a global public health problem. Long-distance truck drivers live apart from their family for long periods of time a lifestyle that favors at-risk behaviors such as unprotected sex with multiple partners and illicit drug use. As data concerning HCV infection in this population are still rare this paper aims to investigate the prevalence genotypes subtypes and the factors associated with HCV infection in long-distance truck drivers in Brazil. A cross-sectional survey was carried out with 641 Brazilian long-truck drivers who were recruited at a major truck stop located at kilometer 1 296 of the BR-153 highway which is considered to be one of the longest roads in Brazil. All individuals were interviewed and their serum samples were tested for the presence of antibodies to HCV anti-HCV by ELISA and immunoblot. Anti-HCV positive samples were tested for HCV RNA by PCR amplification of the 5 NC and NS5B regions and were genotyped using the LiPA assay and nucleotide sequencing respectively. Factors associated with HCV infection were identified with logistic regression. The prevalence of HCV infection was 95 CI . History of blood transfusion sharing of personal hygiene tools illicit drug use and HBV status were factors independently associated with HCV infection in the study population. HCV RNA was detected in 8 9 anti-HCV positive samples in which genotypes 1 n 3 2 n 2 and 3 n 3 were determined by LiPA. Using phylogenetic tree analysis of the NS5B region subtypes 1a n 1 1b n 2 2b n 2 and 3a n 3 were identified. These data show that the prevalence of HCV infection among Brazilian .