tailieunhanh - Báo cáo y học: " Molecular characterization of Chikungunya virus isolates from clinical samples and adult Aedes albopictus mosquitoes emerged from larvae from Kerala, South India"

Molecular characterization of Chikungunya virus isolates from clinical samples and adult Aedes albopictus mosquitoes emerged from larvae from Kerala, South India | Niyas et al. Virology Journal 2010 7 189 http content 7 1 189 VIROLOGY JOURNAL SHORT REPORT Open Access Molecular characterization of Chikungunya virus isolates from clinical samples and adult Aedes albopictus mosquitoes emerged from larvae from Kerala South India 1 1 2 1 Kudukkil P Niyas Rachy Abraham Ramakrishnan Nair Unnikrishnan Thomas Mathew Sajith Nair Anoop Manakkadan 1 Aneesh Issac1 Easwaran Sreekumar1 Abstract Chikungunya virus CHIKV an arthritogenic alphavirus is transmitted to humans by infected Aedes Ae. aegypti and mosquitoes. In the study reverse-transcription PCR RT PCR and virus isolation detected CHIKV in patient samples and also in adult mosquitoes that was derived from larvae collected during a chikun-gunya CHIK outbreak in Kerala in 2009. The CHIKV strains involved in the outbreak were the East Central and South African ECSA genotype that had the E1 A226V mutation. The viral strains from the mosquitoes and CHIK patients from the same area showed a close relationship based on phylogenetic analysis. Genetic characterization by partial sequencing of non-structural protein 2 nsP2 378 bp envelope E1 505 bp and E2 428 bp identified one critical mutation in the E2 protein coding region of these CHIKV strains. This novel non-conservative mutation L210Q consistently present in both human and mosquito-derived samples studied was within the region of the E2 protein amino acids E2 200-220 that determines mosquito cell infectivity in many alpha viruses. Our results show the involvement of Ae. albopictus in this outbreak in Kerala and appearance of CHIKV with novel genetic changes. Detection of virus in adult mosquitoes emerged in the laboratory from larvae also points to the possibility of transovarial transmission TOT of mutant CHIKV strains in mosquitoes. Findings Chikungunya virus CHIKV is an alphavirus of the Togaviridae family and is an important re-emerging pathogen. It has been responsible for major .