tailieunhanh - Báo cáo y học: " In vitro evaluation of marine-microorganism extracts for anti-viral activity"

In vitro evaluation of marine-microorganism extracts for anti-viral activity | Yasuhara-Bell et al. Virology Journal 2010 7 182 http content 7 1 182 VIROLOGY JOURNAL RESEARCH Open Access In vitro evaluation of marine-microorganism extracts for anti-viral activity Jarred Yasuhara-Bell1 2 Yongbo Yang2 Russell Barlow3 Hank Trapido-Rosenthal3 Yuanan Lu1 2 Abstract Viral-induced infectious diseases represent a major health threat and their control remains an unachieved goal due in part to the limited availability of effective anti-viral drugs and measures. The use of natural products in drug manufacturing is an ancient and well-established practice. Marine organisms are known producers of pharmacological and anti-viral agents. In this study a total of 20 extracts from marine microorganisms were evaluated for their antiviral activity. These extracts were tested against two mammalian viruses herpes simplex virus HSV-1 and vesicular stomatitis virus VSV using Vero cells as the cell culture system and two marine virus counterparts channel catfish virus CCV and snakehead rhabdovirus SHRV in their respective cell cultures CCO and EPC . Evaluation of these extracts demonstrated that some possess antiviral potential. In sum extracts 162M 4 258M 1 298M 4 313 2 331M 2 367M 1 and 397 1 appear to be effective broad-spectrum antivirals with potential uses as prophylactic agents to prevent infection as evident by their highly inhibitive effects against both virus types. Extract 313 2 shows the most potential in that it showed significantly high inhibition across all tested viruses. The samples tested in this study were crude extracts therefore the development of antiviral application of the few potential extracts is dependent on future studies focused on the isolation of the active elements contained in these extracts. Background Viruses cause many important diseases in humans with viral-induced emerging and re-emerging infectious diseases representing a major health threat to the public. In addition viruses can also infect livestock and .