tailieunhanh - Báo cáo y học: "Sustained viral load and late death in Rag2-/mice after influenza A virus infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Sustained viral load and late death in Rag2-/mice after influenza A virus infection | Wu et al. Virology Journal 2010 7 172 http content 7 1 172 VIROLOGY JOURNAL SHORT REPORT Open Access Sustained viral load and late death in Rag2- -mice after influenza A virus infection 12 2 1 Haiya Wu Verena Haist Wolfgang Baumgartner Klaus Schughart Abstract The importance of the adaptive immune response for secondary influenza infections and protection from a lethal challenge after vaccination has been well documented. However some controversy still exists concerning the specific involvement of B and T cells during a primary infection. Here we have followed the survival weight loss viral load and lung pathology in Rag2v knock-out mice after infection with influenza A virus H1N1 . Infected wild type mice initially lost weight early after infection but then cleared the virus and recovered. Rag2v mice however showed similar weight loss kinetics in the early stages after infection but weight loss continued post infection and culminated in death. In contrast to wild type mice Ragy- mice were not able to clear the virus despite an increased inflammatory response. Furthermore they did not recruit virus-specific lymphocytes into the lung in the later stages after infection and exhibited sustained pulmonary lesions. Findings The essential role of the adaptive immune system for a secondary protective immune response after primary infection or vaccination has been demonstrated previously for review . 1 2 . In primary infected mice depletion of CD8 and NK cells caused increased death 3 whereas depletion of CD4 cells resulted in delayed viral clearance but survival of infected mice 4 . p2 m knock-out mice which are deficient in CD8 cells survived infection and cleared the virus 5 . However the specific role of B cells during primary infection is still somewhat controversial. Mice lacking mature B cells pMT- - were more susceptible to virus infections whereas pMT- - mice primed with a sub-lethal dose survived a subsequent infection 6 . Although mice .