tailieunhanh - Báo cáo y học: "Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis | Zhang et al. Virology Journal 2010 7 168 http content 7 1 168 VIROLOGY JOURNAL RESEARCH Open Access Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis 1 f 2f 1 f Shunchuan Zhang Guangpeng Ma Jun Xiang Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia3 Qihui Luo3 Zhengli Chen3 Xiaoyue Chen1 3 4 Abstract Background Duck viral enteritis which is caused by duck enteritis virus DEV causes significant economic losses in domestic and wild waterfowls because of the high mortality and low egg production rates. With the purpose of eliminating this disease and decreasing economic loss in the commercial duck industry researching on glycoprotein K gK of DEV may be a new kind of method for preventing and curing this disease. Because glycoproteins project from the virus envelope as spikes and are directly involved in the host immune system and elicitation of the host immune responses and also play an important role in mediating infection of target cells the entry into cell for free virus and the maturation or egress of virus. The gK is one of the major envelope glycoproteins of DEV. However little information correlated with gK is known such as antigenic and functional characterization. Results Bioinformatic predictions revealed that the expression of the full-length gK gene fgK in a prokaryotic system is difficult because of the presence of suboptimal exon and transmembrane domains at the C-terminal. In this study we found that the fgK gene might not be expressed in a prokaryotic system in accordance with the bioinformatic predictions. Further we successfully used bioinformatics tools to guide the prokaryotic expression of the gK gene by designing a novel truncated gK gene tgK . These findings indicated that bioinformatics provides theoretical data for target gene expression and saves time for our research. The recombinant tgK protein tgK was expressed and purified by immobilized metal affinity