tailieunhanh - Báo cáo y học: "A systematic approach for the identification of novel, serologically reactive recombinant Varicella-Zoster Virus (VZV) antigens"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A systematic approach for the identification of novel, serologically reactive recombinant Varicella-Zoster Virus (VZV) antigens | Vizoso Pinto et al. Virology Journal 2010 7 165 http content 7 1 165 VIROLOGY JOURNAL RESEARCH Open Access A systematic approach for the identification of novel serologically reactive recombinant Varicella-Zoster Virus VZV antigens 1 f 2f 2 2 2 Maria G Vizoso Pinto Klaus-Ingmar Pfrepper Tobias Janke Christina Noelting Michaela Sander 3 14 1 1 1 Angelika Lueking Juergen Haas Hans Nitschko Gundula Jaeger Armin Baiker Abstract Background Varicella-Zoster virus causes chickenpox upon primary infection and shingles after reactivation. Currently available serological tests to detect VZV-specific antibodies are exclusively based on antigens derived from VZV-infected cells. Results We present a systematic approach for the identification of novel serologically reactive VZV antigens. Therefore all VZV open reading frames were cloned into a bacterial expression vector and checked for small scale recombinant protein expression. Serum profiling experiments using purified VZV proteins and clinically defined sera in a microarray revealed 5 putative antigens ORFs 1 4 14 49 and 68 . These were rearranged in line format and validated with pre-characterized sera. Conclusions The line assay confirmed the seroreactivity of the identified antigens and revealed its suitability for VZV serodiagnostics comparable to commercially available VZV-ELISA. Recombinant ORF68 gE proved to be an antigen for high-confidence determination of VZV serostatus. Furthermore our data suggest that a serological differentiation between chickenpox and herpes zoster may be possible by analysis of the IgM-portfolio against individual viral antigens. Background The Varicella-Zoster virus VZV is a member of the neurotropic alphaherpesvirus subfamily of the Herpes-viridae. VZV causes varicella chickenpox during primary infection and may cause herpes zoster shingles as secondary disease after reactivation from latency. Varicella can be considered as a harmless childhood disease. However severe .