tailieunhanh - Báo cáo y học: "Bovine ISG15: an antiviral and inducible protein in BIV infected fetal bovine lung cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Bovine ISG15: an antiviral and inducible protein in BIV infected fetal bovine lung cells | Liu et al. Virology Journal 2010 7 134 http content 7 1 134 VIROLOGY JOURNAL SHORT REPORT Open Access Bovine ISG15 an antiviral and inducible protein in BIV infected fetal bovine lung cells Chang Liu 1 2 Xin Li1 Xue Yao1 Xiaohong Kong2 Wentao Qiao1 and Yunqi Geng 1 Abstract Bovine ISG15 bISG15 is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant understanding the properties of bISG15 capable of being inducible in fetal bovine lung FBL cells upon infection of bovine immunodeficiency virus BIV is of significant importance. In this study we investigated the expression of bISG15 in poly I C treated FBL cells. The increased expression of bISG15 was observed and the inhibition of BIV replication was also detected in FBL cells. Elimination of bISG15 expression by small interfering RNA reversed the bISG15 mediated inhibition of BIV replication. These findings demonstrate that bISG15 plays an important role in inhibition of the BIV replication in FBL cells. Furthermore real-time PCR and western blot assay revealed that bISG15 s expression can also be induced in BIV infected FBL cells. Taken together bISG15 is an antiviral and inducible protein in BIV infected FBL cells. Finding Type I IFNs binding to cell type specific receptors can induce the synthesis of a plethora of proteins 1 many of which play crucial roles in the antiviral response 2 . ISG15 was one of the most strongly induced proteins. Thirty years past since ISG15 was first found 3 whereas its antiviral function was reported recently 4 . ISG15 a 15-kDa ubiquitin-like protein can be covalently conjugated to target proteins for post-translational modification through a series of steps similar to ubiquitin modification 5 . ISG15 and its conjugation modification play roles in processes including regulation of IFN signaling innate immunity anti-viral infections pregnancy and carcinogenesis 4 . Interestingly ISG15 and enzymes .