tailieunhanh - Báo cáo y học: " Decreased HIV diversity after allogeneic stem cell transplantation of an HIV-1 infected patient: a case report"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Decreased HIV diversity after allogeneic stem cell transplantation of an HIV-1 infected patient: a case report | Kamp et al. Virology Journal 2010 7 55 http content 7 1 55 VIROLOGY JOURNAL CASE REPORT Open Access Decreased HIV diversity after allogeneic stem cell transplantation of an HIV-1 infected patient a case report 1 Ỷ 2Ỷ 4Ỷ 1 1 1 Christel Kamp Timo Wolf Ignacio G Bravo Benjamin Kraus Birgit Krause Britta Neumann Gudrun Winskowsky 1 Alexander Thielen5 Albrecht Werner3 Barbara S Schnierle1 Abstract The human immunodeficiency virus type 1 HIV-1 coreceptor use and viral evolution were analyzed in blood samples from an HIV-1 infected patient undergoing allogeneic stem cell transplantation SCT . Coreceptor use was predicted in silico from sequence data obtained from the third variable loop region of the viral envelope gene with two software tools. Viral diversity and evolution was evaluated on the same samples by Bayesian inference and maximum likelihood methods. In addition phenotypic analysis was done by comparison of viral growth in peripheral blood mononuclear cells and in a CCR5 R5 -deficient T-cell line which was controlled by a reporter assay confirming viral tropism. In silico coreceptor predictions did not match experimental determinations that showed a consistent R5 tropism. Anti-HIV directed antibodies could be detected before and after the SCT. These preexisting antibodies did not prevent viral rebound after the interruption of antiretroviral therapy during the SCT. Eventually transplantation and readministration of anti-retroviral drugs lead to sustained increase in CD4 counts and decreased viral load to undetectable levels. Unexpectedly viral diversity decreased after successful SCT. Our data evidence that only R5-tropic virus was found in the patient before and after transplantation. Therefore blocking CCR5 receptor during stem cell transplantation might have had beneficial effects and this might apply to more patients undergoing allogeneic stem cell transplantation. Furthermore we revealed a scenario of HIV-1 dynamic different from the .