tailieunhanh - Báo cáo y học: " Highly pathogenic avian influenza A virus H5N1 NS1 protein induces caspase-dependent apoptosis in human alveolar basal epithelial cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Highly pathogenic avian influenza A virus H5N1 NS1 protein induces caspase-dependent apoptosis in human alveolar basal epithelial cells | Zhang et al. Virology Journal 2010 7 51 http content 7 1 51 VIROLOGY JOURNAL RESEARCH Open Access Highly pathogenic avian influenza A virus H5N1 NS1 protein induces caspase-dependent apoptosis in human alveolar basal epithelial cells X - 71 1t 2 2 3 1 Chuanfu Zhang 1 Yutao Yang 1 t Xiaowei Zhou 1 Xuelin Liu Hongbin Song Yuxian He Peitang Huang Abstract Background It is widely considered that the multifunctional NS1 protein of influenza A viruses contributes significantly disease pathogenesis by modulating a number of virus and host-cell processes but it is highly controversial whether this non-structural protein is a proapoptotic or antiapoptotic factor in infected cells. Results NS1 protein of influenza A chicken Jilin 2003 virus a highly pathogenic H5N1 strain could induce apoptosis in the carcinomic human alveolar basal epithelial cells A549 by electron microscopic and flow cytometric analyses. NS1 protein-triggered apoptosis in A549 cells is via caspase-dependent pathway. Conclusions Influenza A virus NS1 protein serves as a strong inducer of apoptosis in infected human respiratory epithelial cells and plays a critical role in disease pathogenesis. Background The influenza A virus which contains eight segmented and negative-stranded RNAs as its genome is a globally important human and animal respiratory pathogen responsible for both seasonal flu outbreaks and periodic world-wide pandemics. In recent years the highly pathogenic avian influenza A virus H5N1 has been frequently transmitted to human and caused a mortality rate of 30 raising serious worldwide concern about a severe influenza pandemic. Although considerable efforts the mechanism accounting for the severity of human H5N1 infection remains elusive. It has been demonstrated that influenza viruses can induce apoptosis in numerous cell types both in vivo 1-3 and in vitro 4-12 . Recently the apoptosis was observed among the alveolar epithelial cells of two patients who died of .