tailieunhanh - báo cáo khoa học: " Identification of genes associated with growth cessation and bud dormancy entrance using a dormancy-incapable tree mutant"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of genes associated with growth cessation and bud dormancy entrance using a dormancy-incapable tree mutant | Jimenez et al. BMC Plant Biology 2010 10 25 http 1471-2229 10 25 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of genes associated with growth cessation and bud dormancy entrance using a dormancy-incapable tree mutant Sergio Jiménez1 Zhigang Li1 Gregory L Reighard1 Douglas G Bielenberg1 2 Abstract Background In many tree species the perception of short days SD can trigger growth cessation dormancy entrance and the establishment of a chilling requirement for bud break. The molecular mechanisms connecting photoperiod perception growth cessation and dormancy entrance in perennials are not clearly understood. The peach Prunus persica L. Batsch evergrowing evg mutant fails to cease growth and therefore cannot enter dormancy under SD. We used the evg mutant to filter gene expression associated with growth cessation after exposure to SD. Wild-type and evg plants were grown under controlled conditions of long days 16 h 8 h followed by transfer to SD 8 h 16 h for eight weeks. Apical tissues were sampled at zero one two four and eight weeks of SD and suppression subtractive hybridization was performed between genotypes at the same time points. Results We identified 23 up-regulated genes in the wild-type with respect to the mutant during SD exposure. We used quantitative real-time PCR to verify the expression of the differentially expressed genes in wild-type tissues following the transition to SD treatment. Three general expression patterns were evident one group of genes decreased at the time of growth cessation after 2 weeks in SD another that increased immediately after the SD exposure and then remained steady and another that increased throughout SD exposure. Conclusions The use of the dormancy-incapable mutant evg has allowed us to reduce the number of genes typically detected by differential display techniques for SD experiments. These genes are candidates for involvement in the signalling pathway leading from photoperiod .