tailieunhanh - báo cáo khoa học: " A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L.) genome"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L.) genome | Hong et al. BMC Plant Biology 2010 10 17 http 1471-2229 10 17 BMC Plant Biology RESEARCH ARTICLE Open Access A SSR-based composite genetic linkage map for the cultivated peanut Arachis hypogaea L. genome Yanbin Hong1 Xiaoping Chen1 2 3 Xuanqiang Liang1 Haiyan Liu1 Guiyuan Zhou1 Shaoxiong Li1 Shijie Wen1 C Corley Holbrook4 Baozhu Guo2 Abstract Background The construction of genetic linkage maps for cultivated peanut Arachis hypogaea L. has and continues to be an important research goal to facilitate quantitative trait locus QTL analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts in which only 135 simple sequence repeat SSR markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat SSR markers derived primarily from peanut genomic sequences expressed sequence tags ESTs and by data mining sequences released in GenBank. Results Three recombinant inbred lines RILs populations were constructed from three crosses with one common female parental line Yueyou 13 a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs 146 124 and 64 primer pairs markers were polymorphic in these populations respectively and used in genotyping these RIL populations. Individual linkage maps were constructed from each of the three populations and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 .