tailieunhanh - báo cáo khoa học: " Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis) | Foster et al. BMC Plant Biology 2010 10 13 http 1471-2229 10 13 BMC Plant Biology RESEARCH ARTICLE Open Access Single nucleotide polymorphisms for assessing genetic diversity in castor bean Ricinus communis IdFFrdx T Wctor1 I Allan2 A noc p r ban3 Pabln n 73 4 5 oc Pa al3 4 6 P A I I kirl cnn7 Jeiney I roster Gerard J Allan Agnes P Chan Pablo D Rabil lowicz Jacques Ravel Paul J Jackson Paul Keim1 Abstract Background Castor bean Ricinus communis is an agricultural crop and garden ornamental that is widely cultivated and has been introduced worldwide. Understanding population structure and the distribution of castor bean cultivars has been challenging because of limited genetic variability. We analyzed the population genetics of R. communis in a worldwide collection of plants from germplasm and from naturalized populations in Florida . To assess genetic diversity we conducted survey sequencing of the genomes of seven diverse cultivars and compared the data to a reference genome assembly of a widespread cultivar Hale . We determined the population genetic structure of 676 samples using single nucleotide polymorphisms SNPs at 48 loci. Results Bayesian clustering indicated five main groups worldwide and a repeated pattern of mixed genotypes in most countries. High levels of population differentiation occurred between most populations but this structure was not geographically based. Most molecular variance occurred within populations 74 followed by 22 among populations and 4 among continents. Samples from naturalized populations in Florida indicated significant population structuring consistent with local demes. There was significant population differentiation for 56 of 78 comparisons in Florida pairwise population jPT values p . Conclusion Low levels of genetic diversity and mixing of genotypes have led to minimal geographic structuring of castor bean populations worldwide. Relatively few lineages occur and these are widely distributed. Our