tailieunhanh - báo cáo khoa học: " Analysis of a c0t-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of a c0t-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris | Zakrzewski et al. BMC Plant Biology 2010 10 8 http 1471-2229 10 8 BMC Plant Biology RESEARCH ARTICLE Open Access Analysis of a c0t-1 library enables the targeted identification of minisatellite and satellite families in Beta vulgaris Falk Zakrzewski1 Torsten WenkeH Daniela Holtgrawe2 Bernd Weisshaar2 Thomas Schmidt1 Abstract Background Repetitive DNA is a major fraction of eukaryotic genomes and occurs particularly often in plants. Currently the sequencing of the sugar beet Beta vulgaris genome is under way and knowledge of repetitive DNA sequences is critical for the genome annotation. We generated a c0t-1 library representing highly to moderately repetitive sequences for the characterization of the major B. vulgaris repeat families. While highly abundant satellites are well-described minisatellites are only poorly investigated in plants. Therefore we focused on the identification and characterization of these tandemly repeated sequences. Results Analysis of 1763 c0t-1 DNA fragments providing 442 kb sequence data shows that the satellites pBV and pEV are the most abundant repeat families in the B. vulgaris genome while other previously described repeats show lower copy numbers. We isolated 517 novel repetitive sequences and used this fraction for the identification of minisatellite and novel satellite families. Bioinformatic analysis and Southern hybridization revealed that minisatellites are moderately to highly amplified in B. vulgaris. FISH showed a dispersed localization along most chromosomes clustering in arrays of variable size and number with exclusion and depletion in distinct regions. Conclusion The c0t-1 library represents major repeat families of the B. vulgaris genome and analysis of the c0t-1 DNA was proven to be an efficient method for identification of minisatellites. We established so far the broadest analysis of minisatellites in plants and observed their chromosomal localization providing a background for the annotation of

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