tailieunhanh - báo cáo khoa học: " Identification of a Rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa × O. glaberrima introgression lines"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of a Rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa × O. glaberrima introgression lines | Gutierrez et al. BMC Plant Biology 2010 10 6 http 1471-2229 10 6 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of a Rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa X O. glaberrima introgression lines Andrés Gonzalo Gutierrez 1 Silvio James Carabalí1 Olga Ximena Giraldo1 César Pompilio Martinez1 Fernando Correa1 3 Gustavo Prado1 Joe Tohme1 Mathias Lorieux1 2 Abstract Background Developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. Chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for QTL detection and breeding programs. Results We built a set of 64 chromosome segment substitution lines carrying contiguous chromosomal segments of African rice Oryza glaberrima MG12 acc. IRGC103544 in the genetic background of Oryza sativa ssp. tropical japonica cv. Caiapó . Well-distributed simple-sequence repeats markers were used to characterize the introgression events. Average size of the substituted chromosomal segments in the substitution lines was about 10 cM and covered the whole donor genome except for small regions on chromosome 2 and 4. Proportions of recurrent and donor genome in the substitution lines were and respectively. The remaining corresponded to heterozygotes and missing data. Strong segregation distortion was found on chromosomes 3 and 6 indicating the presence of interspecific sterility genes. To illustrate the advantages and the power of quantitative trait loci QTL detection using substitution lines a QTL detection was performed for scored traits. Transgressive segregation was observed for several traits measured in the population. Fourteen QTLs for plant height tiller number per plant panicle length sterility percentage 1000-grain weight and grain yield were .