tailieunhanh - báo cáo khoa học: " Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora | Baldo et al. BMC Plant Biology 2010 10 1 http 1471-2229 10 1 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars Malus X domestica with Erwinia amylovora a lol CA3 IMíOrdli4 I- í-íìrrd lr5 I R .iccotr4 IdorH Q A Id A inc-Hd2 i 11 c t A AA K lalniox 1 Angela Baido Jay L Noieiii Robeit E raiieiiji Caioie L Bassett neib S Aidwinckie iviickaei Mainoy Abstract Background The necrogenic enterobacterium Erwinia amylovora is the causai agent of the fire biight FB disease in many Rosaceaespecies including appie and pear. During the infection process the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatibie bacteria-piant interaction. No resistance mechanism to E. amylovora in host piants has yet been characterized recent work has identified some moiecuiar events which occur in resistant and or susceptibie host interaction with E. amylovora In order to understand the mechanisms that characterize responses to FB differential expressed genes were identified by cDNA-AFLP anaiysis in resistant and susceptibie appie genotypes after inocuiation with E. amylovora. Results cDNA were isoiated from susceptibie and resistant appie tissues coiiected 2 h and 48 h after chaiienge with a viruient E. amylovora strain or mock buffer inocuiated. To identify differential expressed transcripts eiectrophoretic banding patterns were obtained from cDNAs. In the AFLP experiments and showed different patterns of expression including genes specificaiiy induced not induced or repressed by E. amylovora. In totai 190 ESTs differential expressed between and were identified using 42 pairs of AFLP primers. cDNA-AFLP anaiysis of giobai EST expression in a resistant and a susceptibie appie genotype identified different major ciasses of genes. EST sequencing data showed that genes iinked to resistance .