tailieunhanh - Báo cáo khoa học: The subcellular organization of strictosidine biosynthesis in Catharanthus roseus epidermis highlights several trans-tonoplast translocations of intermediate metabolites

Catharanthus roseussynthesizes a wide range of valuable monoterpene indole alkaloids, some of which have recently been recognized as func-tioning in plant defence mechanisms. More specifically, in aerial organ epidermal cells, vacuole-accumulated strictosidine displays a dual fate, being either the precursor of all monoterpene indole alkaloids after export from the vacuole. | IFEBS Journal The subcellular organization of strictosidine biosynthesis in Catharanthus roseus epidermis highlights several trans-tonoplast translocations of intermediate metabolites Gregory Guirimand1 Anthony Guihur1 Olivia Ginis1 Pierre Poutrain1 Francois Hericourt2 Audrey Oudin1 Arnaud Lanoue1 Benoit St-Pierre1 Vincent Burlat1 f and Vincent Courdavault1 1 Université Francois Rabelais de Tours EA2106 Biomolécules et Biotechnologies Vegetales IFR 135 Imagerie fonctionnelle Tours France 2 Universite d Orleans EA1207 Laboratoire de Biologie des Ligneux et Grandes Cultures and INRA USC1328 Arbres et Reponses aux Contraintes Hydriques et Environnementales ARCHE Orleans France Keywords alkaloid bimolecular fluorescence complementation Catharanthus roseus methyltransferase strictosidine Correspondence V. Courdavault Universite de Tours -EA2106 Biomolẽcules et Biotechnologies Vegetales UFR des Sciences et Techniques 37200 Tours France Fax 33 247 27 66 60 Tel 33 247 36 69 88 E-mail Present addresses Universite de Toulouse UPS UMR 5546 Surfaces Cellulaires et Signalisation chez les Vegetaux Castanet-Tolosan France ỊCNRS UMR 5546 Castanet-Tolosan France Received 5 October 2010 revised 2 December 2010 accepted 16 December 2010 doi Catharanthus roseus synthesizes a wide range of valuable monoterpene indole alkaloids some of which have recently been recognized as functioning in plant defence mechanisms. More specifically in aerial organ epidermal cells vacuole-accumulated strictosidine displays a dual fate being either the precursor of all monoterpene indole alkaloids after export from the vacuole or the substrate for a defence mechanism based on the massive protein cross-linking which occurs subsequent to organelle membrane disruption during biotic attacks. Such a mechanism relies on a physical separation between the vacuolar strictosidine-synthe-sizing enzyme and the nucleus-targeted enzyme catalyzing its .