tailieunhanh - báo cáo khoa học: " Gene-based microsatellites for cassava (Manihot esculenta Crantz): prevalence, polymorphisms, and cross-taxa utility"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Gene-based microsatellites for cassava (Manihot esculenta Crantz): prevalence, polymorphisms, and cross-taxa utility | BMC Plant Biology BioMed Central Research article Gene-based microsatellites for cassava Manihot esculenta Crantz prevalence polymorphisms and cross-taxa utility Adebola AJ Raji1 James V Anderson2 Olufisayo A Kolade1 3 Chike D Ugwu1 Alfred GO Dixon1 and Ivan L Ingelbrecht 1 Open Access Address International Institute of Tropical Agriculture IITA Oyo Road Ibadan Nigeria 2USDA-Agricultural Research Service Biosciences Research Laboratory 1605 Albrecht Blvd. Fargo ND 58105-5674 USA and 3Africa Rice Center WARDA 01 BP 2031 Cotonou Benin Email Adebola AJ Raji - araji@ James V Anderson - Olufisayo A Kolade - Alfred GO Dixon - adixon@ Ivan L Ingelbrecht - iingelbrecht@ Corresponding author Published II September 2009 Received 24 May 2009 BMC Plant Biology 2009 9 118 doi 1471-2229-9-118 Accepted 11 September 2009 This article is available from http 1471-2229 9 118 2009 Raji et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Cassava Manihot esculenta Crantz a starchy root crop grown in tropical and subtropical climates is the sixth most important crop in the world after wheat rice maize potato and barley. The repertoire of simple sequence repeat SSR markers for cassava is limited and warrants a need for a larger number of polymorphic SSRs for germplasm characterization and breeding applications. Results A total of 846 putative microsatellites were identified in silico from an 8 577 cassava unigene set with an average density of one SSR every 7 kb. One hundred and ninety-two candidate SSRs were screened for polymorphism among a panel of cassava cultivars from Africa Latin America and Asia four wild Manihot .