tailieunhanh - báo cáo khoa học: " Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean | Bolon et al. BMC Plant Biology 2010 10 41 http 1471-2229 10 41 BMC Plant Biology RESEARCH ARTICLE Open Access Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean 1 I z i I I- I o z I z k . 1 f o I k . -1 II I z- r z k- . l .2fc v o z k k3hAi A II A L- m m 3Drỉ A l Ai wr4 A A I i A E I V V I M 5 Yung-Tsi Boion Bindu Joseph Steven B cannon Michelle A Graham Brian w Diers Andrew D Farmer CirantyrM Pi l C 5 O rx I I ioAIHth IQK6 bmQC E Qr ior Ht7 lift Ti 18 Khttan A oolzc3 w x no A Vi 18 Gregory D May Gary J Muehlbauer James E speciu Zheng Jin iu Hainan weeks Wayne w AU Randy c Shoemaker3 Carroll P Vance1 6 Abstract Background The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus QTL on Linkage Group I LG I of soybean Glycine max L. Merrill has a striking effect on seed protein content. Results A soybean near-isogenic line NIL pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than Mbp of genomic sequence on chromosome 20. Using Affymetrix Soy GeneChip and high-throughput Illumina whole transcriptome sequencing platforms 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the Mbp LG I protein QTL region. Conclusions This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs .