tailieunhanh - báo cáo khoa học: " An unedited 1.1 kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An unedited kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility (WA-CMS) system of Oryza sativa L. subsp. indica | Das et al. BMC Plant Biology 2010 10 39 http 1471-2229 10 39 BMC Plant Biology RESEARCH ARTICLE Open Access An unedited kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility WA-CMS system of Oryza sativa L. subsp. indica 1 t 14 1 1 Srirupa Das Supriya Sen Anirban Chakraborty Papia Chakraborti Mrinal K Maiti Asitava Basu Debabrata Basu1 5 Soumitra K Sen1 Abstract Background The application of hybrid rice technology has significantly increased global rice production during the last three decades. Approximately 90 of the commercially cultivated rice hybrids have been derived through three-line breeding involving the use of WA-CMS lines. It is believed that during the 21st century hybrid rice technology will make significant contributions to ensure global food security. This study examined the poorly understood molecular basis of the WA-CMS system in rice. Results RFLPs were detected for atp6 and orfB genes in sterile and fertile rice lines with one copy of each in the mt-genome. The RNA profile was identical in both lines for atp6 but an additional longer orfB transcript was identified in sterile lines. 5 RACE analysis of the long orfB transcript revealed it was 370 bp longer than the normal transcript with no indication it was chimeric when compared to the genomic DNA sequence. cDNA clones of the longer orfB transcript in sterile lines were sequenced and the transcript was determined unedited. Sterile lines were crossed with the restorer and maintainer lines and fertile and sterile F1 hybrids were respectively generated. Both hybrids contained two types of orfB transcripts. However the long transcript underwent editing in the fertile F1 hybrids and remained unedited in the sterile lines. Additionally the editing of the kb orfB transcript cosegregated with fertility restoring alleles in a segregating population of F2 progeny and the presence of unedited long orfB transcripts was detected in the .