tailieunhanh - báo cáo khoa học: " A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs | BMC Plant Biology BioMed Central Open Access Methodology article A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs Klaas Bouwmeester and Francine Govers Address Laboratory of Phytopathology Wageningen University Binnenhaven 5 6709 PD Wageningen and Graduate School Experimental Plant Sciences the Netherlands Email Klaas Bouwmeester - Francine Govers - Corresponding author Published 22 August 2009 Received 8 January 2009 BMC Plant Biology 2009 9 111 doi 1471 -2229-9-1 1 1 Accepted 22 August 2009 This article is available from http 1471-2229 9 111 2009 Bouwmeester and Govers licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Phytophthora species are notorious oomycete pathogens that cause diseases on a wide range of plants. Our understanding how these pathogens are able to infect their host plants will benefit greatly from information obtained from model systems representative for plant-Phytophthora interactions. One attractive model system is the interaction between Arabidopsis and Phytophthora brassicae. Under laboratory conditions Arabidopsis can be easily infected with mycelial plugs as inoculum. In the disease cycle however sporangia or zoospores are the infectious propagules. Since the current P. brassicae zoospore isolation methods are generally regarded as inefficient we aimed at developing an alternative method for obtaining high concentrations of P. brassicae zoospores. Results P. brassicae isolates were tested for pathogenicity on Brussels sprout plants Brassica oleracea .