tailieunhanh - báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation | BMC Plant Biology BioMed Central Research article Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation Angela Rubio Moraga1 Almudena Trapero Mozos1 2 Oussama Ahrazem1 and Lourdes Gomez-Gomez 1 Open Access Address 1Departamento de Ciencia y Tecnología Agroforestal y Genética ETSIA Universidad de Castilla-La Mancha Campus Universitario s n Albacete 02071 Spain and 2Current address Centro Regional de Investigaciones Biomedicas C Almansa 14 Albacete 02006 Spain Email Angela Rubio Moraga - AlmudenaTrapero Mozos - Oussama Ahrazem - Lourdes Gomez-Gomez - Corresponding author Published 20 August 2009 Received 13 March 2009 BMC Plant Biology 2009 9 109 doi 1471 -2229-9-109 Accepted 20 August 2009 This article is available from http 1471-2229 9 109 2009 Moraga et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Flavonol glucosides constitute the second group of secondary metabolites that accumulate in Crocus sativus stigmas. To date there are no reports of functionally characterized flavonoid glucosyltransferases in C. sativus despite the importance of these compounds as antioxidant agents. Moreover their bitter taste makes them excellent candidates for consideration as potential organoleptic agents of saffron spice the dry stigmas of C. sativus. Results Using degenerate primers designed to match the plant secondary product glucosyltransferase PSPG box we cloned a full length cDNA encoding CsGT45 from C. sativus stigmas. This protein showed homology with flavonoid glucosyltransferases. In vitro reactions showed that .