tailieunhanh - báo cáo khoa học: " Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling | BMC Plant Biology BioMed Central Research article Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC villa protein kinase PKV suggests involvement in gibberellin signaling Rosemarie W Hammond and Yan Zhao Open Access Address Molecular Plant Pathology Laboratory United States Department of Agriculture Agricultural Research Service Beltsville Maryland 20705 USA Email Rosemarie W Hammond - Yan Zhao - Corresponding author Published 18 August 2009 Received 9 April 2009 BMC Plant Biology 2009 9 108 doi 1471-2229-9-108 Accepted 18 August 2009 This article is available from http 1471-2229 9 108 2009 Hammond and Zhao licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The serine-threonine protein kinase gene designated pkv protein kinase- viroid induced was previously found to be transcriptionally activated in tomato plants infected with the plant pathogen Potato spindle tuber viroid PSTVd . These plants exhibited symptoms of stunting and abnormal development of leaf root and vascular tissues. The encoded protein PKV is a novel member of the AGC VIIIa group of signal-transducing protein kinases however the role of PKV in plant development is unknown. In this communication we report the phenotypic results of over expression and silencing of pkv in transgenic tobacco. Results Over expression of pkv in Nicotiana tabacum cv. Xanthi tobacco resulted in stunting reduced root formation and delay in flowering phenotypes similar to symptoms of PSTVd infection of tomato. In addition homozygous T2 tobacco plants .