tailieunhanh - báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues | BMC Plant Biology BioMed Central Open Access Uncovering the Arabidopsis thaliana nectary transcriptome investigation of differential gene expression in floral nectariferous tissues Brian W Kram11 Wayne W Xut2 and Clay J Carter 1 Address Department of Biology University of Minnesota Duluth Duluth MN 55812 USA and 2Minnesota Supercomputing Institute University of Minnesota Minneapolis MN 55455 USA Email Brian W Kram - bkram@ Wayne W Xu - wxu@ Clay J Carter - cjcarter@ Corresponding author tEqual contributors Published 15 July 2009 Received 7 April 2009 BMC Plant Biology 2009 9 92 doi 1471-2229-9-92 Accepted 15 July 2009 This article is available from http 1471-2229 9 92 2009 Kram et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Many flowering plants attract pollinators by offering a reward of floral nectar. Remarkably the molecular events involved in the development of nectaries the organs that produce nectar as well as the synthesis and secretion of nectar itself are poorly understood. Indeed to date no genes have been shown to directly affect the de novo production or quality of floral nectar. To address this gap in knowledge the ATH1 Affymetrix GeneChip array was used to systematically investigate the Arabidopsis nectary transcriptome to identify genes and pathways potentially involved in nectar production. Results In this study we identified a large number of genes differentially expressed between secretory lateral nectaries and non-secretory median nectary tissues as well as between mature lateral nectaries post-anthesis and immature lateral nectaries pre-anthesis . Expression within nectaries was also compared to thirteen non-nectary .