tailieunhanh - báo cáo khoa học: " New sources of soybean seed meal and oil composition traits identified through TILLING"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: New sources of soybean seed meal and oil composition traits identified through TILLING | BMC Plant Biology BioMed Central Open Access Research article New sources of soybean seed meal and oil composition traits identified through TILLING Emily C Dierking1 and Kristin D Bilyeu 1 2 Address University of Missouri-Columbia Division of Plant Sciences 110 Waters Hall Columbia MO 65211 USA and 2USDA-ARS Plant Genetics Research Unit 110 Waters Hall Columbia MO 65211 USA Email Emily C Dierking - Kristin D Bilyeu - bilyeuk@ Corresponding author Published 14 July 2009 Received 24 April 2009 BMC Plant Biology 2009 9 89 doi 1471-2229-9-89 Accepted I4 July 2009 This article is available from http 1471-2229 9 89 2009 Dierking and Bilyeu licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Several techniques are available to study gene function but many are less than ideal for soybean. Reverse genetics a relatively new approach can be utilized to identify novel mutations in candidate genes this technique has not produced an allelic variant with a confirmed phenotype in soybean. Soybean raffinose synthase genes and microsomal omega-6 fatty acid desaturase genes were screened for novel alleles in mutagenized soybean populations. Results Four mutations in independent lines were identified in the raffinose synthase gene RS2 two mutations resulted in amino acid mutations and one resulted in an altered seed oligosaccharide phenotype. The resulting phenotype was an increase in seed sucrose levels as well as a decrease in both raffinose and stachyose seed oligosaccharide levels. Three mutations in independent lines were identified in the omega-6 fatty acid desaturase gene FAD2-IA all three mutations resulted in missense amino acid mutations and one .