tailieunhanh - báo cáo khoa học: " Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon | BMC Plant Biology BioMed Central Research article Open Access Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon John P Vogel 1 Metin Tuna2 Hikmet Budak3 Naxin Huo1 Yong Q Gu1 and Michael A Steinwand1 Address 1USDA-ARS Western Regional Research Center Albany CA USA 2Namik Kemal University Department of Field Crops Tekirdag Turkey and 3Sabanci University Biological Science and Bioengineering Program Istanbul Turkey Email John P Vogel - Metin Tuna - mtuna@ Hikmet Budak - budak@ Naxin Huo - Yong Q Gu - Michael A Steinwand - Corresponding author Published 13 July 2009 Received 26 February 2009 BMC Plant Biology 2009 9 88 doi 1471-2229-9-88 Accepted I3 July 2009 This article is available from http 1471-2229 9 88 2009 Vogel et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Brachypodium distachyon Brachypodium is rapidly emerging as a powerful model system to facilitate research aimed at improving grass crops for grain forage and energy production. To characterize the natural diversity of Brachypodium and provide a valuable new tool to the growing list of resources available to Brachypodium researchers we created and characterized a large diverse collection of inbred lines. Results We developed 84 inbred lines from eight locations in Turkey. To enable genotypic characterization of this collection we created 398 SSR markers from BAC end and EST sequences. An analysis of 187 diploid lines from 56 locations with 43 SSR markers showed considerable genotypic diversity. There was some correlation between SSR .