tailieunhanh - báo cáo khoa học: " Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR | BMC Plant Biology BioMed Central Research article Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR Carrie Chambers and Bin Shuai Open Access Address Department of Biological Sciences Wichita State University Wichita KS 67260 USA Email Carrie Chambers - cachambers@ Bin Shuai - Corresponding author Published 10 July 2009 Received 2 March 2009 BMC Plant Biology 2009 9 87 doi 1471-2229-9-87 Accepted I0 July 2009 This article is available from http 1471-2229 9 87 2009 Chambers and Shuai licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background MicroRNAs miRNAs are 22-nt small non-coding RNAs that regulate the expression of specific target genes in many eukaryotes. In higher plants miRNAs are involved in developmental processes and stress responses. Sexual reproduction in flowering plants relies on pollen the male gametophyte to deliver sperm cells to fertilize the egg cell hidden in the embryo sac. Studies indicated that post-transcriptional processes are important for regulating gene expression during pollen function. However we still have very limited knowledge on the involved gene regulatory mechanisms. Especially the function of miRNAs in pollen remains unknown. Results Using miRCURY LNA array technology we have profiled the expression of 70 known miRNAs representing 121 miRBase IDs in Arabidopsis mature pollen and compared the expression of these miRNAs in pollen and young inflorescence. Thirty-seven probes on the array were identified using RNAs isolated from mature pollen 26 of which showed significant differences in expression between mature pollen and inflorescence. Real-time PCR based on TaqMan miRNA .