tailieunhanh - báo cáo khoa học: " Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes | BMC Plant Biology BioMed Central Research article Identification functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes Jens C Gopfert 1 Gillian MacNevin2 Dae-Kyun Ro2 and Otmar Spring1 Open Access Address University of Hohenheim Institute of Botany 210 Garbenstrasse 30 70599 Stuttgart Germany and 2Department of Biological Sciences University of Calgary Calgary T2N 1N4 Canada Email Jens C Gopfert - goepfert@ Gillian MacNevin - gmacnevi@ Dae-Kyun Ro - Otmar Spring - spring@ Corresponding author Published 6 July 2009 BMC Plant Biology 2009 9 86 doi 1471-2229-9-86 Received 21 November 2008 Accepted 6 July 2009 This article is available from http 1471-2229 9 86 2009 Gopfert et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Sesquiterpene lactones are characteristic metabolites of Asteraceae or Compositae which often display potent bioactivities and are sequestered in specialized organs such as laticifers resin ducts and trichomes. For characterization of sunflower sesquiterpene synthases we employed a simple method to isolate pure trichomes from anther appendages which facilitated the identification of these genes and investigation of their enzymatic functions and expression patterns during trichome development. Results Glandular trichomes of sunflower Helianthus annuus L. were isolated and their RNA was extracted to investigate the initial steps of sesquiterpene lactone biosynthesis. Reverse transcription-PCR experiments led to the identification of three sesquiterpene synthases. By combination of in vitro and in vivo characterization of