tailieunhanh - báo cáo khoa học: " DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks | BMC Plant Biology BioMed Central Research article DNA ligase I deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks Wanda M Waterworth 1 Jaroslav Kozak2 Claire M Provost3 Clifford M Bray3 Karel J Angelis2 and Christopher E West1 Open Access Address 1CPS Faculty of Biological Sciences University of Leeds Leeds LS2 9JT UK institute of Experimental Botany AS CR Na Karlovce 1 160 00 Praha 6 Czech Republic and 3Faculty of Life Sciences University of Manchester Oxford Road Manchester M13 9PT UK Email Wanda M Waterworth - fbswmw@ Jaroslav Kozak - kozak@ Claire M Provost - Clifford M Karel J Angelis - angelis@ Christopher E West - Corresponding author Published 26 June 2009 BMC Plant Biology 2009 9 79 doi l47l-2229-9-79 Received 20 January 2009 Accepted 26 June 2009 This article is available from http l47l-2229 9 79 2009 Waterworth et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background DNA ligase enzymes catalyse the joining of adjacent polynucleotides and as such play important roles in DNA replication and repair pathways. Eukaryotes possess multiple DNA ligases with distinct roles in DNA metabolism with clear differences in the functions of DNA ligase orthologues between animals yeast and plants. DNA ligase l present in all eukaryotes plays critical roles in both DNA repair and replication and is indispensable for cell viability. Results Knockout mutants of atligl are lethal. Therefore RNAi lines with reduced levels of AtLIGl .