tailieunhanh - báo cáo khoa học: " Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics | BMC Plant Biology BioMed Central Open Access Research article Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants a valuable tool for functional genomics Bo Jian11 2 3 Wensheng Hou11 Cunxiang Wu1 Bin Liu1 3 Wei Liu1 Shikui Song1 Yurong Bi2 and Tianfu Han 1 Address The National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI Institute of Crop Sciences The Chinese Academy of Agricultural Sciences Beijing 100081 PR China 2School of Life Sciences Lanzhou University Lanzhou Gansu 730000 PR China and 3Current address Department of Biology Norwegian University of Science and Technology Realfagbygget Trondheim NO-7491 Norway Email Bo Jian - jianbo1007@ Wensheng Hou - houwsh@ Cunxiang Wu - wucx@ Bin Liu - Wei Liu - weiliu76@ Shikui Song - ssklss@ Yurong Bi - yrbi@ Tianfu Han - hantf@ Corresponding author fEqual contributors Published 25 June 2009 Received 14 November 2008 BMC Plant Biology 2009 9 78 doi l47l-2229-9-78 Accepted 25 June 2009 This article is available from http l47l-2229 9 78 2009 Jian et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Transgenic approaches provide a powerful tool for gene function investigations in plants. However some legumes are still recalcitrant to current transformation technologies limiting the extent to which functional genomic studies can be performed on. Superroot of Lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. Recently a technique to obtain transgenic L corniculatus plants from Superroot-derived leaves .