tailieunhanh - Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity

As more data are generated from proteome and transcriptome analyses of snake venoms, we are gaining an appreciation of the complexity of the venoms and, to some degree, the various sources of such complexity. How-ever, our knowledge is still far from complete. | FEBS Journal REVIEW ARTICLE Insights into and speculations about snake venom metalloproteinase SVMP synthesis folding and disulfide bond formation and their contribution to venom complexity Jay W. Fox1 and Solange M. T. Serrano2 1 Department of Microbiology University of Virginia Charlottesville VA USA 2 Laboratorio Especialde Toxinologia Aplicada-CAT CEPID Instituto Butantan Sao Paulo Brazil Keywords autolysis disintegrin disulfide bond metalloproteinase post-translational processing proteome snake venom structure SVMP transcriptome Correspondence J. W. Fox Department of Microbiology University of Virginia PO Box 800734 Charlottesville VA 229080734 USA Fax 1 434 982 2514 Tel 1 434 924 0050 E-mail jwf8x@ Received 4 February 2008 revised 27 March 2008 accepted 15 April 2008 doi As more data are generated from proteome and transcriptome analyses of snake venoms we are gaining an appreciation of the complexity of the venoms and to some degree the various sources of such complexity. However our knowledge is still far from complete. The translation of genetic information from the snake genome to the transcriptome and ultimately the proteome is only beginning to be appreciated and will require significantly more investigation of the snake venom genomic structure prior to a complete understanding of the genesis of venom composition. Venom complexity however is derived not only from the venom genomic structure but also from transcriptome generation and translation and perhaps most importantly post-translation modification of the nascent venom proteome. In this review we examine the snake venom metalloproteinases some of the predominant components in viperid venoms with regard to possible synthesis and post-translational mechanisms that contribute to venom complexity. The aim of this review is to highlight the state of our knowledge on snake venom metalloproteinase post-translational processing and to suggest testable hypotheses