tailieunhanh - Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems

Toxin–antitoxin systems, as found in bacterial plasmids and their host chromosomes, play a role in the maintenance of genetic information, as well as in the response to stress. We describe the basic biology of the parD⁄kiskidtoxin–antitoxin system of Escherichia coliplasmid R1, with an emphasis on regulation, toxin activity, potential applications in biotech-nology and its relationships with related toxin–antitoxin systems. | ỊFEBS Journal REVIEW ARTICLE parD toxin-antitoxin system of plasmid R1 - basic contributions biotechnological applications and relationships with closely-related toxin-antitoxin systems Elizabeth Diago-Navarro1 Ana M. Hernandez-Arriaga1 Juan Lopez-Villarejo1 Ana J. Munoz-Gomez1 Monique B. Kamphuis2 Rolf Boelens2 Marc Lemonnier3 and Ramon Diaz-Orejas1 1 Centro de Investigaciones Biologicas CSIC Molecular Microbiology and Infection Biology Madrid Spain 2 NMR Department Utrecht University Utrecht The Netherlands 3 ANTABIO SAS Incubateur Midi-Pyrenees Toulouse France Keywords bacterialRNases gene regulation Kid toxin and Kis antitoxin parD operon plasmid maintenance plasmid R1 toxin-antitoxin systems translation inhibition Correspondence R. Diaz-Orejas Centro de Investigaciones Biologicas CSIC Molecular Microbiology and Infection Biology Ramiro de Maeztu 9 28040 Madrid Spain Fax 3491 5360432 Tel 3491 8373112 E-mail ramondiaz@ Received 1 March 2010 revised 21 May 2010 accepted 27 May 2010 Toxin-antitoxin systems as found in bacterial plasmids and their host chromosomes play a role in the maintenance of genetic information as well as in the response to stress. We describe the basic biology of the parD kiskid toxin-antitoxin system of Escherichia coli plasmid R1 with an emphasis on regulation toxin activity potential applications in biotechnology and its relationships with related toxin-antitoxin systems. Special reference is given to the ccd toxin-antitoxin system of plasmid F because its toxin shares structural homology with the toxin of the parD system. Inter-relations with related toxin-antitoxin systems present in the E. coli chromosome such as the parD homologues chpA mazEF and chpB and the relBE system are also reviewed. The combined structural and functional information that is now available on all these systems as well as the ongoing controversy regarding the role of the chromosomal toxin-antitoxin loci have made this review especially timely. doi .