tailieunhanh - Báo cáo khoa học: The evolutionary relationship between the duplicated copies of the zebrafish fabp11 gene and the tetrapod FABP4, FABP5, FABP8 and FABP9 genes

We describe the structure of a fatty acid-binding protein 11 (fabp11b) gene and its tissue-specific expression in zebrafish. The kb zebrafishfabp11b is the paralog of the previously described zebrafish fabp11a, with a deduced amino acid sequence for Fabp11B exhibiting 65% identity with that of Fabp11A. | ỊFEBS Journal The evolutionary relationship between the duplicated copies of the zebrafish fabpll gene and the tetrapod FABP4 FABP5 FABP8 and FABP9 genes Santhosh Karanth1 Eileen M. Denovan-Wright2 Christine Thisse3 Bernard Thisse3 and Jonathan M. Wright1 1 Department of Biology Dalhousie University Halifax Canada 2 Department of Pharmacology Dalhousie University Halifax Canada 3 Department of Cell Biology University of Virginia Health Sciences Center Charlottesville VA USA Keywords Fabp11 retinaldevelopment spinalcord tandem gene duplication whole genome duplication Correspondence J. M. Wright Department of Biology Dalhousie University Halifax NS Canada B3H 4J1 Fax 1 902 494 3736 Tel 1 902 494 6468 E-mail jmwright@ Website http biology2 Received 23 January 2008 revised 8 March 2008 accepted 9 April 2008 doi We describe the structure of a fatty acid-binding protein 11 fabpllb gene and its tissue-specific expression in zebrafish. The kb zebrafish fabpllb is the paralog of the previously described zebrafish fabplla with a deduced amino acid sequence for Fabp11B exhibiting 65 identity with that of Fabp11A. Whole mount in situ hybridization of a riboprobe to embryos and larvae showed that zebrafish fabpllb transcripts were restricted solely to the retina and were first detected at 24 h postfertilization. In situ hybridization revealed fabpllb transcripts along the spinal cord in adult zebrafish. However the highly sensitive RT-PCR assay detected fabpllb transcripts in the brain heart ovary and eye in adult tissues. By contrast fabplla transcripts had been previously detected in the liver brain heart testis muscle ovary and skin of adult zebrafish. Using the LN54 radiation hybrid panel we assigned zebrafish fabpllb to linkage group 16. Phylogenetic analysis and conserved gene synteny with tetrapod genes indicated that the emergence of two copies of fabpll in the zebrafish genome may have resulted from a fish-specific

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical research
• hemoglobin
• medical knowledge
• medical textbooks
• medical practice
• specialized medicine
• Encyclopedia of Medical Anthropology
• medical
• health
• medical term dictionary
• y tế
• sức khỏe
• Data Mining and Medical Knowledge Management
• Premier Reference Sourse
• statements and documents scientific reports
• medical reports
• medical transfer
• the developing countries
• clinical trials
• medical management
• health care
• Healthcare knowledge management
• Knowledge identification
• Know How and Knowing That cartography
• Knowledge visualization
• Improving knowledge management
• Knowledge workers’ identities
• Evidence from the medical profession
• Knowledge workers
• Medical profession
• Organizational identification
• Knowledge Management
• Data Mining
• Text Mining
• Mapping Medical
• Bioinformatics Challenges
• Digital Libraries
• Medical Informatics
• A Handbook for Medical Teachers
• Medical Teachers
• health education
• health knowledge