tailieunhanh - Báo cáo khoa học: Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein

The human prion protein (PrP) is a glycoprotein with a glycosylphosphat-idylinositol (GPI) anchor at its C-terminus. Here we report alternative splic-ing within exon 2 of the PrPgene (PRNP) in the human glioblastoma cell line T98G. The open reading frame of the alternatively spliced mRNA lacked the GPI anchor signal sequence and encoded a 230 amino acid polypeptide. | ỊFEBS Journal Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein Yutaka Kikuchi1 Tomoshi Kakeya1 Osamu Nakajima2 Ayako Sakai1 Kikuko Ikeda3 Naoto Yamaguchi3 Takeshi Yamazaki4 Ken-ichi Tanamoto4 Haruo Matsuda5 Jun-ichi Sawada2 and Kosuke Takatori1 1 Division of Microbiology National institute of Health Sciences Tokyo Japan 2 Division of Biochemistry and Immunochemistry National institute of Health Sciences Tokyo Japan 3 Department of Molecular Cell Biology Graduate Schoolof PharmaceuticalScience Chiba University Japan 4 Division of Food Additives National institute of Health Sciences Tokyo Japan 5 Laboratory of Immunobiology Graduate Schoolof Biosphere Science Hiroshima University Japan Keywords alternative splicing Creudzfeldt-Jakob disease GPI anchor hypoxia prion Correspondence Y. Kikuchi Division of Microbiology National Institute of Health Sciences 1-18-1 Kamiyoga Setagaya-ku Tokyo 158-8501 Japan Fax 81 3 3707 6950 Tel 81 3 3700 1141 E-mail kikuchi@ Received 30 January 2008 revised 24 March 2008 accepted 7 April2008 doi The human prion protein PrP is a glycoprotein with a glycosylphosphatidylinositol GPI anchor at its C-terminus. Here we report alternative splicing within exon 2 of the PrP gene PRNP in the human glioblastoma cell line T98G. The open reading frame of the alternatively spliced mRNA lacked the GPI anchor signal sequence and encoded a 230 amino acid polypeptide. Its product GPI-anchorless PrP GPI PrPSV was unglycosylated and soluble in non-ionic detergent and was found in the cytosolic fraction. We also detected low levels of alternatively spliced mRNA in human brain and non-neuronal tissues. When long-term passaged T98G cells were placed in a low-oxygen environment alternatively spliced mRNA expression increased and expression of normally spliced PrP mRNA decreased. These findings imply that oxygen tension regulates GPI PrPSV expression in T98G cells. Fatal human prion .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• medical research
• analysis of cytoplasmic
• BMC Plant Biology
• Upland cotton
• Restorer gene
• High throughput sequencing
• Cytoplasmic male sterility
• Plant Biology
• Multi omics analysis
• Brassica napus
• Naturally exists
• Mitochondrial genome functions
• BMC Genomics
• RNA sequencing
• Restorer of fertility
• Sterile phenotype
• Parental genes
• Binding miRNAs
• Triple hybrids
• Gossypium bickii
• Proteomic analysis
• biochemistry
• molecular Biology
• microRNA genes
• hemoglobin
• medical analysis
• environmental medicine
• human disease
• Crystal structure
• biochemical studies
• Gossypium hirsutum L
• Photosensitive genetic male sterile
• Differentially expressed genes
• Transcriptome analysis
• RNA Seq
• mammals
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• scientific research
• Human telomeric
• Structural features
• hormone medicine
• under construction
• cytoplasm
• molecular
• biosynthesis
• genetics
• PPR protein
• Targeted mutagenesis
• Nuclear restorer gene
• Structure function relationship
• Rubber tree
• Hevea brasiliensis
• Genome sequencing
• Anther transcriptome
• Hybrid wheat
• Pistil like
• Skeletal muscle
• Wnt signaling pathway
• Enzyme activity
• Cytoplasmic surface
• Fertility restorer
• Plant breeders
• Nuclear genes