tailieunhanh - báo cáo khoa học: " Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.) | BMC Plant Biology BioMed Central Research article Identification characterization and utilization of unigene derived microsatellite markers in tea Camellia sinensis L. Ram Kumar Sharma 1 Pankaj Bhardwaj1 Rinu Negi1 Trilochan Mohapatra2 and Paramvir Singh Ahuja1 Open Access Address biotechnology Division Institute of Himalayan Bioresource Technology IHBT CSIR Post Box 6 Palampur Himachal Pradesh 176061 India and 2National Research Centre on Plant Biotechnology Indian Agricultural Research Institute IARI New Delhi 110012 India Email Ram Kumar Sharma - mrk_sharma@ PankajBhardwaj - pankajihbt@ Rinu Negi - rinunegi@ Trilochan Mohapatra - tm@ Paramvir Singh Ahuja - psahuja@ Corresponding author Published II May 2009 Received 30 January 2008 BMC Plant Biology 2009 9 53 doi 1471-2229-9-53 Accepted 1 1 May 2009 This article is available from http I47I-2229 9 53 2009 Sharma et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Despite great advances in genomic technology observed in several crop species the availability of molecular tools such as microsatellite markers has been limited in tea Camellia sinensis L. . The development of microsatellite markers will have a major impact on genetic analysis gene mapping and marker assisted breeding. Unigene derived microsatellite UGMS markers identified from publicly available sequence database have the advantage of assaying variation in the expressed component of the genome with unique identity and position. Therefore they can serve as efficient and cost effective alternative markers in such species. Results Considering the multiple advantages of UGMS markers 1 223 unigenes were predicted .