tailieunhanh - báo cáo khoa học: " Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis | BMC Plant Biology BioMed Central Open Access Research article Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis Joon-Woo Ahn1 Brian J Atwell2 and Thomas H Roberts 1 Address Department of Chemistry and Biomolecular Sciences Macquarie University North Ryde NSW 2109 Australia and 2Department of Biological Sciences Macquarie University North Ryde NSW 2109 Australia Email Joon-Woo Ahn - Brian J Atwell - batwell@ Thomas H Roberts - troberts@ Corresponding author Published II May 2009 Received 15 December 2008 BMC Plant Biology 2009 9 52 doi 1471-2229-9-52 Accepted 11 May 2009 This article is available from http I47I-2229 9 52 2009 Ahn et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The complex responses of plants to DNA damage are incompletely understood and the role of members of the serpin protein family has not been investigated. Serpins are functionally diverse but structurally conserved proteins found in all three domains of life. In animals most serpins have regulatory functions through potent irreversible inhibition of specific serine or cysteine proteinases via a unique suicide-substrate mechanism. Plant serpins are also potent proteinase inhibitors but their physiological roles are largely unknown. Results Six Arabidopsis genes encoding full-length serpins were differentially expressed in developing seedlings and mature tissues. Basal levels of AtSRP2 At2g 14540 and AtSRP3 At1g64030 transcripts were highest in reproductive tissues. AtSRP2 was induced 5-fold and AtSRP3 100-fold after exposure of seedlings to low concentrations of methyl .

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