tailieunhanh - báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis | BMC Plant Biology BioMed Central Open Access Methodology article A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis Hirotaka Takahashi1 2 Akira Nozawa1 2 5 Motoaki Seki3 Kazuo Shinozaki4 Yaeta Endo 1 2 5 and Tatsuya Sawasaki 1 2 5 Address 1Cell-Free Science and Technology Research Center Ehime University Matsuyama 790-8577 Japan 2The Venture Business laboratory Ehime University Matsuyama 790-8577 Japan 3Plant Functional Genomics Research Group 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan 4Gene Discovery Research Group RIKEN Plant Science Center 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan and 5RIKEN Genomic Sciences Center 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan Email Hirotaka Takahashi - h-takahashi@ Akira Nozawa-anozawa@ Motoaki Seki - mseki@ Kazuo Shinozaki - sinozaki@ Yaeta Endo - yendo@ Tatsuya Sawasaki - sawasaki@ Corresponding authors Published 6 April 2009 Received 26 December 2008 BMC Plant Biology 2009 9 39 doi 1471-2229-9-39 Accepted 6 April 2009 This article is available from http 1471-2229 9 39 2009 Takahashi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Ubiquitination is mediated by the sequential action of at least three enzymes the El ubiquitin-activating enzyme E2 ubiquitin-conjugating enzyme and E3 ubiquitin ligase proteins. Polyubiquitination of target proteins is also implicated in several critical cellular processes. Although Arabidopsis genome research has estimated more than 1 300 proteins involved in .