tailieunhanh - báo cáo khoa học: " Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachis wild species"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachis wild species | BMC Plant Biology BioMed Central Research article Open Access Utility of EST-derived SSR in cultivated peanut Arachis hypogaea L. and Arachis wild species Xuanqiang Liang 1 Xiaoping Chen1 Yanbin Hong1 Haiyan Liu1 Guiyuan Zhou1 Shaoxiong Li1 and Baozhu Guo2 Address 1Crops Research Institute Guangdong Academy of Agricultural Sciences Wushan 510640 Guangzhou PR China and 2USDA-ARS Crop Protection and Management Research Unit Tifton Georgia USA Email Xuanqiang Liang - Liang-804@ Xiaoping Chen - xpchen@ Yanbin Hong - Hongyanbin1979@ Haiyan Liu - Liu_Haiyan001@ GuiyuanZhou-zhguyu418@ Shaoxiong Li-lishaoxiong@ Baozhu Guo - Corresponding author Published 24 March 2009 Received 13 October 2008 BMC Plant Biology 2009 9 35 doi l47l-2229-9-35 Accepted 24 March 2009 This article is available from http l47l-2229 9 35 2009 Liang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Lack of sufficient molecular markers hinders current genetic research in peanuts Arachis hypogaea L. . It is necessary to develop more molecular markers for potential use in peanut genetic research. With the development of peanut EST projects a vast amount of available EST sequence data has been generated. These data offered an opportunity to identify SSR in ESTs by data mining. Results In this study we investigated 24 238 ESTs for the identification and development of SSR markers. In total 88l SSRs were identified from 780 SSR-containing unique ESTs. On an average one SSR was found per kb of EST sequence with tri-nucleotide motifs being the most abundant followed by di- tetra- hexa- and penta-nucleotide repeat