tailieunhanh - báo cáo khoa học: " Cbf genes of the Fr-A2 allele are differentially regulated between long-term cold acclimated crown tissue of freeze-resistant and – susceptible, winter wheat mutant lines"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Cbf genes of the Fr-A2 allele are differentially regulated between long-term cold acclimated crown tissue of freeze-resistant and – susceptible, winter wheat mutant lines | BMC Plant Biology BioMed Central Research article Open Access Cbf genes of the Fr-A2 allele are differentially regulated between long-term cold acclimated crown tissue of freeze-resistant and -susceptible winter wheat mutant lines Fedora Sutton 1 Ding-Geng Chen2 Xijin Ge2 and Don Kenefick1 Address 1Plant Science Department South Dakota State University Plant Science Building Jackrabbit lane Brookings SD 57007 USA and 2Department of Mathematics and Statistics South Dakota State University Brookings SD 57007 Box 2220 Brookings SD 57007 Office Harding Hall 118 USA Email Fedora Sutton - Ding-Geng Chen - Xijin Ge - Don Kenefick - Corresponding author Published 23 March 2009 Received I December 2008 BMC Plant Biology 2009 9 34 doi 1471-2229-9-34 Accepted 23 March 2009 This article is available from http I47I-2229 9 34 2009 Sutton et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In order to identify genes that might confer and maintain freeze resistance of winter wheat a comparative transcriptome analysis was performed between control and 4 wk cold-acclimated crown tissue of two winter wheat lines that differ in field freeze survival. The lines generated by azide mutagenesis of the winter wheat cultivar Winoka were designated FR 75 survival and FS 30 survival . Using two winter lines for this comparative analysis removed the influence of differential expression of the vernalization genes and allowed our study to focus on Cbf genes located within the Fr-A2 allele independent of the effect of the closely mapped Vrn allele. Results Vernalization genes Vrn-A1 Bl and DI and the transcription .