tailieunhanh - Báo cáo khoa học: Plant nuclear proteomics – inside the cell maestro

The eukaryotic nucleus is highly dynamic and complex, containing several subcompartments, several types of DNA and RNA, and a wide range of proteins. Interactions between these components within the nucleus form part of a complex regulatory system that is only partially understood. | ỊFEBS Journal REVIEW ARTICLE Plant nuclear proteomics - inside the cell maestro Matthias Erhardt1 Iwona Adamska1 and Octavio Luiz Franco2 1 Department of Plant Physiology and Biochemistry University of Konstanz Germany 2 Centre for Proteomic and BiochemicalAnalyses Post-Graduate Programme in Genomic Sciences and Biotechnology Catholic University of Brasilia Brazil Keywords cell culture cellular proteomics plant nuclear bodies plant nuclear proteome proteome comparison Correspondence O. L. Franco SGAN Quadra 916 Modulo B Av. W5 Norte - Asa Norte Brasilia-DF Brazil Fax 55 61 3347 4797 Tel 55 61 3448 7220 E-mail ocfranco@ Received 15 May 2010 revised 21 June 2010 accepted 23 June 2010 doi The eukaryotic nucleus is highly dynamic and complex containing several subcompartments several types of DNA and RNA and a wide range of proteins. Interactions between these components within the nucleus form part of a complex regulatory system that is only partially understood. Rapid improvements in proteomics applications have led to a better overall determination of nucleus protein content thereby enabling researchers to focus more thoroughly on protein-protein interactions structures activities and even post-translational modifications. Whereas proteomics research is quite advanced in animals yeast and Escherichia coli plant proteomics is only at the initial phase especially when a single organelle is targeted. For this reason this review focuses on the plant nucleus and its unique properties. The most recent data on the nuclear subproteome will be presented as well as a comparison between the nuclei of plants and mammals. Finally this review also evaluates proteins identified by proteomics that may contribute to our understanding of how the plant nucleus works and proposes novel proteomics technologies that could be utilized for investigating the cell maestro. Introduction The nucleus is the most prominent structure within a .