tailieunhanh - Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages

Obesity is a low-grade chronic inflammatory disease associated with an increased number of macrophages (adipose tissue macrophages) in adipose tissue. Within the adipose tissue, adipose tissue macrophages are the major source of visfatin⁄pre-B-cell colony-enhancing factor⁄nicotinamide phos-phoribosyl transferase. | ễFEBS Journal Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages Thérèse Hèrvée Mayi1 2 3 4 Christian Duhem1 2 3 4 Corinne Copin1 2 3 4 Mohamed Amine Bouhlel1 2 3 4 Elena Rigamonti1 2 3 4 Francois Pattou1 5 6 Bart Staels1 2 3 4 and Giulia Chinetti-Gbaguidi1 2 3 4 1 Univ Lille Nord de France France 2 Inserm Lille France 3 UDSL Lille France 4 Institut Pasteur de Lille France 5 Service de Chirurgie Générale et Endocrinienne Centre Hospitalier Regionalet Universitaire de Lille France 6 Inserm ERIT-M 0106 Faculte de Medecine Lille France Keywords adipocytokines inflammation macrophages nuclear receptors visfatin Correspondence Bart Staels Inserm UR 1011 Institut Pasteur de Lille 1 rue du Professeur Calmette BP 245 Lille 59019 France Fax 33 3 20 87 73 60 Tel 33 3 20 87 73 88 E-mail Received 15 January 2010 revised 27 April 2010 accepted 3 June 2010 doi Obesity is a low-grade chronic inflammatory disease associated with an increased number of macrophages adipose tissue macrophages in adipose tissue. Within the adipose tissue adipose tissue macrophages are the major source of visfatin pre-B-cell colony-enhancing factor nicotinamide phosphoribosyl transferase. The nuclear receptor peroxisome proliferator-acti-vated receptor gamma PPARy exerts anti-inflammatory effects in macrophages by inhibiting cytokine production and enhancing alternative differentiation. In this study we investigated whether PPARy modulates visfatin expression in murine bone marrow-derived macrophage and human primary human resting macrophage classical macrophage alternative macrophage or adipose tissue macrophage macrophage models and pre-adipocyte-derived adipocytes. We show that synthetic PPARy ligands increase visfatin gene expression in a PPARy-dependent manner in primary human resting macrophages and in adipose tissue macrophages but not in adipocytes. The threefold increase of visfatin mRNA was