tailieunhanh - Nanotechnology Science and Computation Part 13

Tham khảo tài liệu 'nanotechnology science and computation part 13', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Computing with Hairpins and Secondary Structures of DNA 305 Experiment 1 spectrophotoscopy The proper behavior of the AND gate was observed as in Fig. 6. Due to the weak interaction between input2 and leader without the existence of inputl output was observed slightly after input2 was put in. However a much stronger signal was observed after input1 was inserted. The quantity of output produced by the gate is about half of the total quantify. Experiments 1 and 2 gel electrophoresis The results are depicted in Fig. 7. One can clearly observe the bands for the complexes output leader output leader input1 and leader input1 input2. The latter two are in close proximity. In Lane 6 output leader input1 input2 the band for output is clearly observed while in Lane 5 output leader input2 the band is observable but much weaker. Unfortunately since the band for output next observed in Lane 16 output next is very close to those for output leader input1 and leader input1 input2 it cannot be judged whether output and next hybridize together in Lane 10 output leader next input1 input2 . However comparing Lanes 10 and 6 the band for output observed in Lane 6 disappears in Lane 10 so it can be deduced that output successfully hybridizes with next. Moreover the smeared wide band in Lane 16 is also observed in Lane 10. Experiment 2 spectrophotoscopy Figure 8 a shows the graph of the observed fluorescence intensity after next input2 and input1 are put to the mixture of output and leader in this order. Since the fluorescence intensity does not change after next is put we can conclude that the AND gate and the next gate next do not interact before the inputs to the AND gate are given. Figure 8 b shows the graphs for all the six orders. Experiment 3 spectrophotoscopy Since the competing gate shares the first input with the original gate it is expected that about half of the first input hybridizes with the competing gate and thus the output of the original gate decreases. Figure 9 shows .