tailieunhanh - Nanotechnology Science and Computation part 3

Tham khảo tài liệu 'nanotechnology science and computation part 3', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | DNA Nanotechnology an Evolving Field 43 Fig. 5. Programmable self-assembly of streptavidin protein arrays templated by 4x4 DNA nanogrids. a Schematic drawing of the DNA-nanogrid-scaffolded assembly of streptavidin. Left the DNA nanogrids a biotin group labeled by a red letter B is incorporated into one of the T4 loops at the center of each tile. Right binding of streptavidin represented by a blue tetramer to biotin will lead to a protein nanoarray on a DNA lattice. Note that this is a one-tile system so that every tile in the lattice contains a biotin group. b AFM image of a self-assembled protein array a scale bar is shown at the bottom of the image. c Strand structure of 4 x 4 tiles A blue and B pink for the construction of the 2D nanogrids of a two-tile system. Complementary sticky-end pairs are labeled as n and n . d e Schematic drawings of the AB tile self-assembly and subsequent binding of streptavidin onto an A B lattice and an A B lattice respectively. Biotin groups and streptavidin molecules are represented by smaller red dots and larger yellow cylinders respectively. f g AFM images obtained after binding of streptavidin to the bare DNA nanogrids A B and A B respectively. The scan sizes of both AFM images are 1 m x 1 m with 150 nm x 150 nm zoom-in insets. self-assembly may provide a viable solution. The periodicities and interparticle spacings defined by DNA nanostructures are readily adjustable with subnanometer spatial resolution. This level of precision provides exquisite control in the construction of rationally defined 2D and 3D nanoscale assemblies. The development of self-assembled DNA nanoscaffolds to organize functional nanomaterials is still in a very early stage. Recently Kiehl and coworkers 22 reported the use of 2D DX lattice as a template to organize 5 nm 44 H. Yan Y. Liu Fig. 6. Aptamer-directed self-assembly of thrombin protein on a triple-crossover DNA tile. a The red strand contains a thrombin-binding aptamer sequence. The TX tiles .