tailieunhanh - Báo cáo khoa học: Serine-arginine protein kinases: a small protein kinase family with a large cellular presence

Serine-arginine protein kinases (SPRKs) constitute a relatively novel subfamily of serine-threonine kinases that specifically phosphorylate serine residues residing in serine-arginine⁄arginine-serine dipeptide motifs. Fifteen years of research subsequent to the purification and cloning of human SRPK1 as a SR splicing factor-phosphorylating protein have lead to the accumulation. | ẶFEBS Journal REVIEW ARTICLE Serine-arginine protein kinases a small protein kinase family with a large cellular presence Thomas Giannakouros1 Eleni Nikolakaki1 Ilias Mylonis2 and Eleni Georgatsou2 1 Laboratory of Biochemistry Department of Chemistry Aristotle University of Thessaloniki Greece 2 Laboratory of Biochemistry Department of Medicine Schoolof Health Sciences University of Thessaly Larissa Greece Keywords LBR metabolic signalling nuclear envelope p53 PGC-1 protamine spermatogenesis splicing SR protein SRPK Correspondence E. Georgatsou Laboratory of Biochemistry Department of Medicine Schoolof Health Sciences University of Thessaly Biopolis 41110 Larissa Greece Fax 30 2410 685545 Tel 30 2410 685581 E-mail egeorgat@ Received 7 July 2010 accepted 26 October 2010 doi Serine-arginine protein kinases SPRKs constitute a relatively novel subfamily of serine-threonine kinases that specifically phosphorylate serine residues residing in serine-arginine arginine-serine dipeptide motifs. Fifteen years of research subsequent to the purification and cloning of human SRPK1 as a SR splicing factor-phosphorylating protein have lead to the accumulation of information on the function and regulation of the different members of this family as well as on the genomic organization of SRPK genes in several organisms. Originally considered to be devoted to constitutive and alternative mRNA splicing SRPKs are now known to expand their influence to additional steps of mRNA maturation as well as to other cellular activities such as chromatin reorganization in somatic and sperm cells cell cycle and p53 regulation and metabolic signalling. Similarly SRPKs were considered to be constitutively active kinases although several modes of regulation of their function have been demonstrated implying an elaborate cellular control of their activity. Finally SRPK gene sequence information from bioinformatics data reveals that SRPK gene homologs exist .