tailieunhanh - Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis

The nuclear pore complex (NPC) is the only gateway for molecular traf-ficking across the nuclear envelope. The NPC is not merely a static nuclear-cytoplasmic transport gate; the functional analysis of nucleoporins has revealed dynamic features of the NPC in various cellular functions, such as mitotic spindle formation and protein modification. | IFEBS Journal Nup358 a nucleoporin functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis Munehiro Asally1 Yoshinari Yasuda2 Masahiro Oka1 2 Shotaro Otsuka3 Shige H. Yoshimura3 Kunio Takeyasu3 and Yoshihiro Yoneda1 2 4 1 Japan Science and Technology Agency Core Research for EvolutionalScience and Technology Department of Frontier Biosciences Graduate Schoolof Frontier Biosciences Osaka University Japan 2 Department of Frontier Biosciences Graduate Schoolof Frontier Biosciences Osaka University Japan 3 Department of Responses to EnvironmentalSignals and Stresses Graduate Schoolof Biostudies Kyoto University Japan 4 Department of Biochemistry and Molecular Biology Graduate Schoolof Medicine Osaka University Japan Keywords NPC remodeling nuclear pore complex nuclear transport Nup358 RanBP2 skeletalmyogenesis Correspondence Y. Yoneda Department of Frontier Biosciences Graduate School of Frontier Biosciences Osaka University 1-3 Yamada-oka Suita Osaka 565-0871 Japan Fax 81 6 6879 4609 Tel 81 6 6879 4606 E-mail yyoneda@ Received 23 August 2010 revised 26 November 2010 accepted 3 December 2010 doi The nuclear pore complex NPC is the only gateway for molecular trafficking across the nuclear envelope. The NPC is not merely a static nuclear-cytoplasmic transport gate the functional analysis of nucleoporins has revealed dynamic features of the NPC in various cellular functions such as mitotic spindle formation and protein modification. However it is not known whether the NPC undergoes dynamic changes during biological processes such as cell differentiation. In the present study we evaluate changes in the expression levels of several nucleoporins and show that the amount of Nup358 RanBP2 within individual NPCs increases during muscle differentiation in C2C12 cells. Using atomic force microscopy we demonstrate structural differences between the cytoplasmic surfaces of .