tailieunhanh - Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding

Recently, a novel uracil-DNA-degrading factor protein (UDE) was identi-fied inDrosophila melanogaster, with homologues only in pupating insects. Its unique uracil-DNA-degrading activity and a potential domain organiza-tion pattern have been described. | IFEBS Journal Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding Angela Bekesi1 Maria Pukancsik1 Peter Haasz1 Lilla Felfoldi1 Ibolya Leveles1 Villo Muha1 Eva Hunyadi-Gulyas2 Anna Erdei3 Katalin F. Medzihradszky2 4 and Beata G. Vertessy1 5 1 Institute of Enzymology BiologicalResearch Centre Hungarian Academy of Sciences Budapest Hungary 2 Proteomics Research Group BiologicalResearch Centre Hungarian Academy of Sciences Szeged Hungary 3 Department of Immunology Eotvos Lorand University of Sciences Budapest Hungary 4 Department of PharmaceuticalChemistry University of California San Francisco USA 5 Department of Applied Biotechnology Budapest University of Technology and Economics Hungary Keywords conformationalstates cotranslational folding RNA-assisted folding RNA binding uracil-DNA-degrading factor Correspondence A. Bekesi and B. G. Vertessy Karolina Street 29 H-1113 Budapest Hungary Fax 36 1 466 5465 Tel 36 1 279 3116 E-mail bekesi@ vertessy@ Website http Received 7 June 2010 revised 29 October 2010 accepted 4 November 2010 doi Recently a novel uracil-DNA-degrading factor protein UDE was identified in Drosophila melanogaster with homologues only in pupating insects. Its unique uracil-DNA-degrading activity and a potential domain organization pattern have been described. UDE seems to be the first representative of a new protein family with unique enzyme activity that has a putative role in insect development. In addition UDE may also serve as potential tool in molecular biological applications. Owing to lack of homology with other proteins with known structure and or function de novo data are required for a detailed characterization of UDE structure and function. Here experimental evidence is provided that recombinant protein is present in two distinct conformers. One of these contains a significant amount of RNA .