tailieunhanh - Báo cáo khoa học: The role of cytochrome P450 monooxygenases in microbial fatty acid metabolism

Cytochrome P450 monooxygenases (P450s) are a diverse collection of enzymes acting on various endogenous and xenobiotic molecules. Most of them catalyse hydroxylation reactions and one group of possible substrates are fatty acids and their related structures. In this minireview, the signifi-cance of P450s in microbial fatty acid conversion is described. | IFEBS Journal MINIREVIEW The role of cytochrome P450 monooxygenases in microbial fatty acid metabolism Inge N. A. Van Bogaert1 Sara Groeneboer2 Karen Saerens1 and Wim Soetaert1 1 Department of Biochemicaland MicrobialTechnology Laboratory of IndustrialBiotechnology and Biocatalysis Ghent University Ghent Belgium 2 Laboratory for Protein Biochemistry and Biomolecular Engineering Ghent University Ghent Belgium Keywords alkanes bacteria biosurfactants cytochrome P450 monooxygenase dicarboxylic acids fatty acids hydroxylation oxylipins polyketides yeast Correspondence I. N. A. Van Bogaert Department of Biochemicaland MicrobialTechnology Laboratory of Industrial Biotechnology and Biocatalysis Faculty of Bioscience Engineering Ghent University Coupure Links 653 B-9000 Ghent Belgium Fax 32 9 264 62 31 Tel 32 9 264 60 34 E-mail Website http Received 22 June 2010 revised 19 August 2010 accepted 16 September 2010 doi Cytochrome P450 monooxygenases P450s are a diverse collection of enzymes acting on various endogenous and xenobiotic molecules. Most of them catalyse hydroxylation reactions and one group of possible substrates are fatty acids and their related structures. In this minireview the significance of P450s in microbial fatty acid conversion is described. Bacteria and yeasts possess various P450 systems involved in alkane and fatty acid degradation and often several enzymes with different activities and specificities are retrieved in one organism. Furthermore P450s take part in the formation of fatty acid-based secondary metabolites. Finally there are a substantial number of microbial P450s displaying activity towards fatty acids but to which no biological role could be assigned despite the often quite intense research. Introduction P450 classification and nomenclature Cytochrome P450s form a vast and divergent family of enzymes. They are heme-thiolate proteins bearing in a hydrophobic pocket a .