tailieunhanh - Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri

Sesbania mosaic virus (SeMV) is a single-stranded positive-sense RNA plant virus belonging to the genus Sobemovirus. The movement protein (MP) encoded by SeMV ORF1 showed no significant sequence similarity with MPs of other genera, but showed 32% identity with the MP of South-ern bean mosaic virus within the Sobemovirus genus. | IFEBS Journal Interaction of Sesbania mosaic virus movement protein with the coat protein - implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri Department of Biochemistry Indian Institute of Science Bangalore India Keywords coat protein protein-protein interaction recombinant movement protein sobemovirus Correspondence H. S. Savithri Department of Biochemistry Indian Institute of Science Bangalore 560012 India Fax 91 8023600814 Tel 91 8022932310 E-mail bchss@ Received 10 June 2010 revised 28 September 2010 accepted 1 November 2010 doi Sesbania mosaic virus SeMV is a single-stranded positive-sense RNA plant virus belonging to the genus Sobemovirus. The movement protein MP encoded by SeMV ORF1 showed no significant sequence similarity with MPs of other genera but showed 32 identity with the MP of Southern bean mosaic virus within the Sobemovirus genus. With a view to understanding the mechanism of cell-to-cell movement in sobemoviruses the SeMV MP gene was cloned over-expressed in Escherichia coli and purified. Interaction of the recombinant MP with the native virus NV was investigated by ELISA and pull-down assays. It was observed that SeMV MP interacted with NV in a concentration- and pH-dependent manner. Analysis of N- and C-terminal deletion mutants of the MP showed that SeMV MP interacts with the NV through the N-terminal 49 amino acid segment. Yeast two-hybrid assays confirmed the in vitro observations and suggested that SeMV might belong to the class of viruses that require MP and NV coat protein for cell-to-cell movement. Structured digital abstract MINT-8050243 p53 uniprotkb P02340 physically interacts MI 0915 with T-Ag uni-protkb P03070 by two hybrid MI 0018 MINT-8050226 MP uniprotkb Q9EB09 physically interacts MI 0915 with CP uni-protkb Q9EB06 by two hybrid MI 0018 Introduction Plants have an elaborate communication system that permits transport of macromolecules from one

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