tailieunhanh - Báo cáo y học: "Differential expression of interferon-induced microRNAs in patients with chronic hepatitis C virus infection treated with pegylated interferon alpha"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential expression of interferon-induced microRNAs in patients with chronic hepatitis C virus infection treated with pegylated interferon alpha | Scagnolari et al. Virology Journal 2010 7 311 http content 7 1 311 VIROLOGY JOURNAL RESEARCH Open Access Differential expression of interferon-induced microRNAs in patients with chronic hepatitis C virus infection treated with pegylated interferon alpha 1 2 2 1 2 3 Carolina Scagnolari Pompea Zingariello Jacopo Vecchiet Carla Selvaggi Delia Racciatti Gloria Taliani Elisabetta Riva4 Eligio Pizzigallo2 Guido Antonelli1 Abstract There have been reports of in-vitro interferon IFN -mediated antiviral activity against the hepatitis C virus HCV through microRNAs miRNAs . The main aim of this study was to evaluate the expression of several miRNAs miR-1 miR-30 miR-128 miR-196 miR-296 in peripheral blood mononuclear cells PBMCs from healthy individuals after in vitro IFN-treatment and in PBMCs from patients with chronic hepatitis C CHC before and 12 hours after the first injection of pegylated IFN alpha. We demonstrated that expression of these miRNAs could be recorded in PBMCs collected from healthy individuals before and after in-vitro IFN alpha treatment. Our analysis revealed that the levels of expression of all miRNAs investigated in patients with CHC were different to those in healthy individuals. When levels of the miRNAs were measured 12 hours after the first IFN injection increases in expression levels of IFN-induced miRNAs were observed in 25-50 of patients depending on the type of miRNA examined. No correlations were observed between HCV viral load alanine aminotransferase status and expression of miRNA. Together these findings suggest that i IFN alpha in-vitro treatment of PBMCs leads to a transcriptional induction of all miR-NAs investigated ii miRNAs can be induced differentially by IFN treatment in patients with HCV. Given the importance of miRNAs in defending the host against virus infections it is possible that IFN-induced miRNAs may represent an important determinant of the clinical outcome of IFN therapy in HCV infection. Introduction .