tailieunhanh - Báo cáo y học: " Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients | Cao et al. Virology Journal 2010 7 299 http content 7 1 299 VIROLOGY JOURNAL RESEARCH Open Access Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients 71 z- 1Ỷ 2Ỷ 3 1 3 2 1 Zhiliang Cao Lifeng Liu Lanying Du Chao Zhang Shibo Jiang Taisheng Li Yuxian He Abstract Background The spike S protein of SARS-CoV not only mediates receptor-binding but also induces neutralizing antibodies. We previously identified the receptor-binding domain RBD of S protein as a major target of neutralizing antibodies in animal models and thus proposed a RBD-based vaccine. However the antigenicity and immunogenicity of RBD in humans need to be characterized. Results Two panels of serum samples from recovered SARS patients were included and the antibody responses against the RBD were measured by ELISA and micro-neutralization assays. We found that the RBD of S protein induced potent antibody responses in the recovered SARS patients and RBD-specific antibodies could persist at high titers over three year follow-up. Furthermore affinity purified anti-RBD antibodies possessed robust neutralizing activity. Conclusion The RBD of SARS-CoV is highly immunogenic in humans and mediates protective responses and RBD-based vaccines and diagnostic approaches can be further developed. Background The global outbreak of severe acute respiratory syndrome SARS caused by a novel coronavirus SARS-CoV resulted in more than 8 000 cases with a fatality rate of about 10 . Impressively the rapid spread of SARS-CoV made a great impact on public health and social-economic stability. It is thought that SARS-CoV might originate from its natural reservoir bats and transmit to humans through an intermediate such as palm civets and raccoon dogs and no one can exclude the possibility of its recurrence 1 . SARS-CoV is an enveloped positive-stranded RNA virus and its crown -like spike S protein has two major biological functions 1 .