tailieunhanh - Báo cáo y học: "Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma | Redaelli et al. Virology Journal 2010 7 298 http content 7 1 298 VIROLOGY JOURNAL SHORT REPORT Open Access Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma Marco Redaelli1 Carla Mucignat-Caretta1 Andrea Cavaggioni1 Antonio Caretta3 Domenico D Avella2 Luca Denaro2 Sandro Cavirani4 Gaetano Donofrio4 Abstract The application of gene therapy for malignant gliomas is still under study and the use of specific vectors represents an important contribution. Here we investigated bovine herpesvirus 4 BoHV-4 which is non-pathogenic if injected into the rodent brain. We show that the vector can infect mouse rat and human glioma cell lines and primary cultures obtained from human glioblastoma in vitro. BoHV-4 was injected into a tumour grown in rat brain. Although virus expression was scattered across the tumour mass it was mainly located in the peripheral area of larger gliomas. These data support BoHV-4 as a candidate vector for glioma treatment. Findings Gene therapy for the selective treatment of brain tumours is intriguing particularly given the limited efficacy of currently available therapeutic options. In thirteen studies that performed clinical trials with gene therapy results showed an increase in mean survival time ranging from months to months 1 . The optimization of potential vectors is essential for clinical effectiveness of cancer gene therapy. Bovine herpesvirus 4 BoHV-4 belongs to the Herpes-viridae family gamma-herpesviridae subfamily 2 . The monocyte macrophage lineage is one of the sites of persistence of infection in cattle a natural host and in experimental hosts the rabbit 3 . BoHV-4 is able to replicate in a broad range of host species both in vivo and in vitro 4 . BoHV-4 replicates and causes a cyto-pathic effect CPE in a large number of immortalized cell lines and primary cultures 3 5 6 . Although BoHV-4 is not considered a neurotropic virus it has been isolated in peripheral and central